ON THE REGULATORY CAPABILITIES OF THYROID MAST CELLS IN THIAMAZOLE MODEL OF HYPOTHYROIDISM AND INFRARED LASER EXPOSURE
I.V. Smelova ¹, E.S. Golovneva ¹, ², T.G. Kravchenko ¹, V.I. Petukhova ¹, ²
DOI: 10.22138/2500-0918-2021-18-1-20-28
¹ GBUZ «Multidisciplinary Center of Laser Medicine», Chelyabinsk, Russian Federation;
² FSBEI HE South-Ural State Medical University MOH Russia, Chelyabinsk, Russian Federation
Abstract. The regulatory effect of mast cells on the state of thyroid gland in hypothyroidism and laser therapy remains unclear. Aim: to study the secretory processes of mast cells in relationship with the indicators of functional activity of thyroid gland. Materials and methods. Experimental groups: (55 rats) 1) intact rats, 2) hypothyroidism (thiamazole 25mg/kg) 3) hypothyroidism and 0.5W laser exposure, 4) hypothyroidism and 2.0W laser exposure. Histological samples of the thyroid gland were removed on the 1, 7, and 30 days. Histological sections were stained with toluidine blue. Morphometric data analysis included descriptive statistics and non-parametric tests (Mann Whitney, Spearman correlation coefficient). Results. The increase in the granular saturation of mast cells and the average histochemical coefficient was observed in the hypothyroidism group, the degranulation index increased by day 30. After 0.5 W laser exposure, there was a decrease in the granular content in mast cells and an increase in the degranulation index; the granular saturation increased by day 30. After 2.0 W laser exposure, the content of granules in mast cells decreased on day 1, and on days 7 and 30 it was higher than in the hypothyroidism group; the degranulation index decreased by day 30. The correlation was revealed between the indicators of granule accumulation in a mast cell, the index of mast cell degranulation, the thyroid epithelium height, and relative vascular area. Conclusions. The synthesis processes prevailed over secretion for mastocytes in thiamazole hypothyroidism. 0.5 W laser exposure was more effective for stimulation of the secretory processes in mast cells compared to 2.0 W exposure. The secretory activity of mast cells was associated with the functional activity of thyroid gland, which confirms their regulatory role in tissue repair after thiamazole induced hypothyroidism modeling.
Keywords: mast cells, secretion, laser, hypothyroidism, thyroid gland
There is no conflict of interest.
Contact details of the corresponding author:
Elena S. Golovneva
micron30@mail.ru
Received 02.02.2021
For citation:
Smelova I.V., Golovneva E.S., Kravchenko T.G., Petukhova V.I. On the Regulatory Capabilities of Thyroid Mast Cells in Thiamazole Model of Hypothyroidism and Infrared Laser Exposure. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2021, Vol. 18, no. 1, pp. 20–28. DOI: 10.22138/2500-0918-2021-18-1-20-28 (In Russ)
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Authors
Irina V. Smelova
Multidisciplinary Center of Laser Medicine
Researcher science
287 Pobedy Ave., Chelyabinsk Russian Federation 454021
spiral.siv@mail.ru
Elena S. Golovneva
Multidisciplinary Center of Laser Medicine
Dr. Sci. (Med.), docent, deputy director for research
287 Pobedy Ave. Chelyabinsk Russian Federation 454021
South-Ural State Medical University MOH Russia
Professor of the department of normal physiology name of acad. Y. M. Zacharov
64 Vorovskogo str. Chelyabinsk Russian Federation 454092
micron30@mail.ru
Tatyana G. Kravchenko
Multidisciplinary Center of Laser Medicine
Cand. Sci. (Biol.), senior researcher
287 Pobedy Ave. Chelyabinsk Russian Federation 454021
bossa_nova@list.ru
Victorya I. Petuсhova
Multidisciplinary Center of Laser Medicine
Cand.Sci. (Med), researcher science
287 Pobedy Ave. Chelyabinsk Russian Federation 454021
South-Ural State Medical University MOH Russia
Docent of the department of normal physiology name of acad. Y. M. Zacharov
64 Vorovskogo str. Chelyabinsk Russian Federation 454092
75vika@rambler.ru
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)